Djokovic, bidding to hold all four Grand Slams simultaneously for the second time in his career, had no such dramas as he followed Osaka on to Court Suzanne Lenglen.
There had been speculation that the 27-year-old, a late inclusion in the squad, would miss the game for health and fitness reasons after sitting out the last four one day internationals.
Hosts England made 311 for eight after being sent into bat by Du Plessis before bowling out the South Africans for 207 to start their campaign with an emphatic 104-run victory at The Oval.
Top female referee Aimee Barret-Theron broke new ground when she became the first woman to referee a Currie Cup game in 2018. Since then she has gone on to bigger things and matches.
Interestingly, Imran Tahir bowled the first ball of the World Cup in a surprise move from Proteas skipper Faf du Plessis.
South Africa will happy with their first 50 overs at the World Cup after restricting England to 311-8 in their allotted 50 overs.
Hashim Amla had made five when he missed an intended pull off the fast bowler, the ball crashing into the grille of his helmet after he was beaten for sheer speed.
Chasing a competitive but very doable target of 312, set by the home team, South Africa fell short in familiar fashion with a mix of soft dismissals and a runout for good measure.
Eden Hazard scored twice and then admitted it was his parting gift as the Belgian's brace helped Chelsea sweep away Arsenal to win 4-1 in the Europa League final in Baku.